Vitamin D, 25-Hydroxy
What it measures
Serum 25-hydroxyvitamin D level — the standard marker for vitamin D status. Distinguishes deficiency (<20 ng/mL), insufficiency (20–29), and sufficiency (≥30).
When it’s ordered
Suspected deficiency (fatigue, bone pain, muscle weakness), osteoporosis workup, malabsorption disorders, chronic kidney disease, post-bariatric surgery.
Medicare coverage
Medicare judges coverage case by case under its medical-necessity rules. Medicare has no national rule for this test. The local Medicare contractor in your state may have its own rules. A routine check with no symptom or condition behind it is the kind of order that gets questioned.
Typical price
Medicare allowable around $40–45. Commercial cash prices range from $50 to $300.
One thing to watch
Ask your doctor to write the symptom or condition on the order, such as bone disease or a problem absorbing vitamins. Check the reason before the draw.
Look up the current Medicare allowable for Vitamin D, 25-Hydroxy →
Rates and coverage rules shown apply to Original Medicare (Fee-for-Service) only. Medicare Advantage plans may differ. See our data sources →